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cell culture human mesenchymal stem cells  (ATCC)


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    ATCC cell culture human mesenchymal stem cells
    Cell Culture Human Mesenchymal Stem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 530 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+mesenchymal+stem+cells/Mesenchymal+Stem+Cell+Basal+Medium+for+Adipose%2C+Umbilical+and+Bone+Marrow-derived+MSCs/pmc12541612__mmc1-4-0-7
    Average 99 stars, based on 530 article reviews
    cell culture human mesenchymal stem cells - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: Dissecting the effects of 223 Radium on the bone microenvironment
    Article Snippet: .. Cell Culture Human mesenchymal stem cells (hMSCs, ATCC) were cultured in Minimum Essential Medium (MEM 1X, Corning) supplemented with 17% fetal bovine serum (FBS, Corning), Basal Medium Eagle vitamins (1X, Sigma), non-essential amino acids (1X, Gibco), penicillin and streptomycin (both 100 μg/mL, Cytiva), and 1% sodium pyruvate (1 mM, Gibco). .. To induce osteoblastic differentiation, hMSCs were cultured in osteogenic medium composed of Dulbecco’s Modified Eagle’s Medium (DMEM 1X, Corning) supplemented with 10% FBS (Corning), penicillin and streptomycin (both 100 μg/mL, Cytiva), 50 μg/mL Lascorbic acid (Sigma), 10 mM β-glycerophosphate (Sigma), 0.1 μM dexamethasone (Sigma), and 1% sodium pyruvate (1 mM, Gibco) for 3 weeks.

    Article Title: Novel Sensing Technique for Stem Cells Differentiation Using Dielectric Spectroscopy of Their Proteins.
    Article Snippet: .. Cell Culture Human mesenchymal stem cells (hMSCs, ATCC, Manassas, VA, USA) and human osteogenic sarcoma cells (Saos-2 cells; Korea Cell Line Bank, Seoul, Republic of Korea) were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F-12 50/50 (DMEM/F12; Gibco, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco) in a humidified incubator at 37 °C with 5% CO2. ..

    Modification:

    Article Title: Novel Sensing Technique for Stem Cells Differentiation Using Dielectric Spectroscopy of Their Proteins.
    Article Snippet: .. Cell Culture Human mesenchymal stem cells (hMSCs, ATCC, Manassas, VA, USA) and human osteogenic sarcoma cells (Saos-2 cells; Korea Cell Line Bank, Seoul, Republic of Korea) were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F-12 50/50 (DMEM/F12; Gibco, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco) in a humidified incubator at 37 °C with 5% CO2. ..



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    Image Search Results


    (A) Human myotube senescent model. To induce the senescence of human MSCs, the cells were treated with doxorubicin for 24 hours and then removed. After 14 days, the conditioned medium of the d-Sen MSC culture was added to human myotubes differentiated from myoblasts (Hu5) for 7 days. (B) A representative Hoechst and γH2AX staining images of human MSCs treated with or without doxorubicin (scale bar, 250 μm). (C) Quantification of fluorescence intensity (γH2AX/Hoechst). (D) Gene expression of senescence-related markers by real-time PCR. Culture of human MSCs with or without doxorubicin. ** p < 0.01. (E) Gene expression of SASP markers by real-time PCR. Culture of human MSCs with or without doxorubicin. **p < 0.01. (F) Representative image of Hu5 cells differentiation. (scale bar, 500 μm). (G) Gene expression of muscle differentiation markers by real-time PCR. The days indicate as duration of culturing of Hu5 cells with DM. *p < 0.05, ** p < 0.01. Statistical analyses were conducted using the student’s t -test.

    Journal: PLOS One

    Article Title: An in vitro model to study molecular pathogenesis of sarcopenia established by a SASP-dependent human myotube culture

    doi: 10.1371/journal.pone.0326968

    Figure Lengend Snippet: (A) Human myotube senescent model. To induce the senescence of human MSCs, the cells were treated with doxorubicin for 24 hours and then removed. After 14 days, the conditioned medium of the d-Sen MSC culture was added to human myotubes differentiated from myoblasts (Hu5) for 7 days. (B) A representative Hoechst and γH2AX staining images of human MSCs treated with or without doxorubicin (scale bar, 250 μm). (C) Quantification of fluorescence intensity (γH2AX/Hoechst). (D) Gene expression of senescence-related markers by real-time PCR. Culture of human MSCs with or without doxorubicin. ** p < 0.01. (E) Gene expression of SASP markers by real-time PCR. Culture of human MSCs with or without doxorubicin. **p < 0.01. (F) Representative image of Hu5 cells differentiation. (scale bar, 500 μm). (G) Gene expression of muscle differentiation markers by real-time PCR. The days indicate as duration of culturing of Hu5 cells with DM. *p < 0.05, ** p < 0.01. Statistical analyses were conducted using the student’s t -test.

    Article Snippet: Human Mesenchymal Stem Cells (MSCs) from Takara Bio were cultured with Mesenchymal Stem Cell Growth Medium 2 (Takara Bio) in early passages and then cultured to be acclimated in 4.5g/L high glucose Dulbecco modified Eagle medium (DMEM; Gibco) containing 10% fetal bovine serum (FBS; JRH Biosciences) and 1% penicillin/streptomycin (FUJIFILM Wako Pure Chemical Corporation).

    Techniques: Staining, Fluorescence, Gene Expression, Real-time Polymerase Chain Reaction